Two-dimensional electronic spectroscopy (2DES) is a powerful tool to study the dynamics of complex molecular systems. Polarization control in 2DES allows enhancement of selective signals, providing information about the transition dipole orientations and rotational motion. In this work, we discuss an implementation of polarization-resolved 2DES (p-2DES) in the pump-probe beam geometry that simultaneously acquires signals under parallel and perpendicular pump-probe polarizations. This approach is general, as these two sets of signals can be combined as desired to obtain 2DES spectra of various polarization schemes, such as magic angle, cross-peak specific, diagonal-peak enhancing, and anisotropy. We demonstrate the approach using (1) phthalocyanine, a molecular system with two orthogonal electronic transitions and (2) the photosynthetic light-harvesting complex II, which exhibits a highly congested spectrum with many concurrent energy transfer processes. We show how the polarization schemes can help resolve hidden spectral and kinetic features. This implementation can help to streamline the use of p-2DES and provide more insights into the structural and dynamical properties of complex systems.
@article{nguyen_implementation_2026,title={An {Implementation} of {General} {Polarization}-{Resolved} {Two}-{Dimensional} {Electronic} {Spectroscopy}},volume={27},issn={1439-7641},url={https://onlinelibrary.wiley.com/doi/abs/10.1002/cphc.202500903},doi={10.1002/cphc.202500903},language={en},number={11},urldate={2026-06-08},journal={ChemPhysChem},author={Nguyen, Hoang Long and Ong, Nelson Kim Soon and Jana, Sanjib and Prasad, Sachin and Do, Thanh Nhut and Caffarri, Stefano and Tan, Howe-Siang},year={2026},keywords={photosynthesis, ultrafast spectroscopy, 2D spectroscopy, energy transfer, polarization},pages={e202500903},}
Directly Measuring the Connectivity between Isoenergetic Light-Harvesting Antennas in Plant Photosystem II at Physiological Temperature
Hoang Long Nguyen, Kai Zhong, Thanh Nhut Do, and 6 more authors
The Journal of Physical Chemistry Letters, Jan 2026
@article{nguyen_directly_2026,title={Directly {{Measuring}} the {{Connectivity}} between {{Isoenergetic Light-Harvesting Antennas}} in {{Plant Photosystem II}} at {{Physiological Temperature}}},author={Nguyen, Hoang Long and Zhong, Kai and Do, Thanh Nhut and Akhtar, Parveen and Lambrev, Petar H. and Jansen, Thomas L. C. and Knoester, Jasper and Caffarri, Stefano and Tan, Howe-Siang},year={2026},month=jan,journal={The Journal of Physical Chemistry Letters},volume={17},number={2},pages={605--614},issn={1948-7185, 1948-7185},doi={10.1021/acs.jpclett.5c02240},urldate={2026-01-25},copyright={https://doi.org/10.15223/policy-029},langid={english},}
2025
Correlated Vibrational Coherence and Spectral Diffusion Analysis of Multi-Level Systems Using Two-Dimensional Electronic Spectroscopy
Sanjib Jana, Sachin Prasad, Hoang Long Nguyen, and 2 more authors
@article{jana_correlated_2025,title={Correlated Vibrational Coherence and Spectral Diffusion Analysis of Multi-Level Systems Using Two-Dimensional Electronic Spectroscopy},author={Jana, Sanjib and Prasad, Sachin and Nguyen, Hoang Long and Le, Duc Viet and Tan, Howe-Siang},year={2025},month=apr,journal={The Journal of Chemical Physics},volume={162},number={16},pages={164311},issn={0021-9606},doi={10.1063/5.0268218},urldate={2025-09-26},}
Application of the Time-Domain Multichromophoric Fluorescence Resonant Energy Transfer Method in the NISE Programme
Kai Zhong, Vesna Erić, Hoang Long Nguyen, and 5 more authors
Journal of Chemical Theory and Computation, Jan 2025
@article{zhong_application_2025,title={Application of the {{Time-Domain Multichromophoric Fluorescence Resonant Energy Transfer Method}} in the {{NISE Programme}}},author={Zhong, Kai and Eri{\'c}, Vesna and Nguyen, Hoang Long and {van Adrichem}, Kim E. and {ten Hoven}, Gijsbert A. H. and Manrho, Marick and Knoester, Jasper and Jansen, Thomas L. C.},year={2025},month=jan,journal={Journal of Chemical Theory and Computation},volume={21},number={1},pages={254--266},publisher={American Chemical Society},issn={1549-9618},doi={10.1021/acs.jctc.4c01135},urldate={2025-01-19},}
2024
Inter-Subunit Energy Transfer Processes in a Minimal Plant Photosystem II Supercomplex
Hoang Long Nguyen, Thanh Nhut Do, Kai Zhong, and 6 more authors
@article{nguyen_inter-subunit_2024,title={Inter-Subunit Energy Transfer Processes in a Minimal Plant Photosystem {{II}} Supercomplex},author={Nguyen, Hoang Long and Do, Thanh Nhut and Zhong, Kai and Akhtar, Parveen and Jansen, Thomas L. C. and Knoester, Jasper and Caffarri, Stefano and Lambrev, Petar and Tan, Howe-Siang},year={2024},month=feb,journal={Science Advances},volume={10},number={8},pages={eadh0911},publisher={American Association for the Advancement of Science},doi={10.1126/sciadv.adh0911},urldate={2024-02-24},}
Coarse-Grained Approach to Simulate Signatures of Excitation Energy Transfer in Two-Dimensional Electronic Spectroscopy of Large Molecular Systems
Kai Zhong, Hoang Long Nguyen, Thanh Nhut Do, and 3 more authors
Journal of Chemical Theory and Computation, Jul 2024
@article{zhong_coarse-grained_2024,title={Coarse-{{Grained Approach}} to {{Simulate Signatures}} of {{Excitation Energy Transfer}} in {{Two-Dimensional Electronic Spectroscopy}} of {{Large Molecular Systems}}},author={Zhong, Kai and Nguyen, Hoang Long and Do, Thanh Nhut and Tan, Howe-Siang and Knoester, Jasper and Jansen, Thomas L. C.},year={2024},month=jul,journal={Journal of Chemical Theory and Computation},volume={20},number={14},pages={6111--6124},publisher={American Chemical Society},issn={1549-9618},doi={10.1021/acs.jctc.4c00413},urldate={2024-10-21},}
2023
Measuring the Ultrafast Spectral Diffusion and Vibronic Coupling Dynamics in CdSe Colloidal Quantum Wells Using Two-Dimensional Electronic Spectroscopy
Hoang Long Nguyen, Thanh Nhut Do, Emek G. Durmusoglu, and 6 more authors
@article{nguyen_measuring_2023,type={Journal {{Article}}},title={Measuring the {{Ultrafast Spectral Diffusion}} and {{Vibronic Coupling Dynamics}} in {{CdSe Colloidal Quantum Wells}} Using {{Two-Dimensional Electronic Spectroscopy}}},author={Nguyen, Hoang Long and Do, Thanh Nhut and Durmusoglu, Emek G. and Izmir, Merve and Sarkar, Ritabrata and Pal, Sougata and Prezhdo, Oleg V. and Demir, Hilmi Volkan and Tan, Howe-Siang},year={2023},journal={ACS Nano},volume={17},number={3},pages={2411--2420},issn={1936-0851 1936-086X},doi={10.1021/acsnano.2c09606},}
An Efficient Time-Domain Implementation of the Multichromophoric Förster Resonant Energy Transfer Method
Kai Zhong, Hoang Long Nguyen, Thanh Nhut Do, and 3 more authors
@article{zhong_efficient_2023,type={Journal {{Article}}},title={An Efficient Time-Domain Implementation of the Multichromophoric {{F\"orster}} Resonant Energy Transfer Method},author={Zhong, Kai and Nguyen, Hoang Long and Do, Thanh Nhut and Tan, Howe-Siang and Knoester, Jasper and Jansen, Thomas L. C.},year={2023},journal={The Journal of Chemical Physics},volume={158},number={6},pages={064103},issn={0021-9606 1089-7690},doi={10.1063/5.0136652},}
2022
Two-Dimensional Electronic Spectroscopy of the Qx to Qy Relaxation of Chlorophylls a in Photosystem II Core Complex
Thanh Nhut Do, Hoang Long Nguyen, Stefano Caffarri, and 1 more author
@article{do_two-dimensional_2022,type={Journal {{Article}}},title={Two-Dimensional Electronic Spectroscopy of the {{Qx}} to {{Qy}} Relaxation of Chlorophylls a in Photosystem {{II}} Core Complex},author={Do, Thanh Nhut and Nguyen, Hoang Long and Caffarri, Stefano and Tan, Howe-Siang},year={2022},journal={The Journal of Chemical Physics},volume={156},number={14},pages={145102},issn={0021-9606 1089-7690},doi={10.1063/5.0079500},}
Ultrafast Excitation Energy Transfer Dynamics in the LHCII–CP29–CP24 Subdomain of Plant Photosystem II
Thanh Nhut Do, Hoang Long Nguyen, Parveen Akhtar, and 6 more authors
@article{do_ultrafast_2022,type={Journal {{Article}}},title={Ultrafast {{Excitation Energy Transfer Dynamics}} in the {{LHCII}}--{{CP29}}--{{CP24 Subdomain}} of {{Plant Photosystem II}}},author={Do, Thanh Nhut and Nguyen, Hoang Long and Akhtar, Parveen and Zhong, Kai and Jansen, Thomas L. C. and Knoester, Jasper and Caffarri, Stefano and Lambrev, Petar H. and Tan, Howe-Siang},year={2022},journal={The Journal of Physical Chemistry Letters},volume={13},number={19},pages={4263--4271},issn={1948-7185 1948-7185},doi={10.1021/acs.jpclett.2c00194},}
2021
An Exciton Dynamics Model of Bryopsis Corticulans Light-Harvesting Complex II
Hoang Long Nguyen, Thanh Nhut Do, Parveen Akhtar, and 6 more authors
@article{nguyen_exciton_2021,type={Journal {{Article}}},title={An {{Exciton Dynamics Model}} of {{Bryopsis}} Corticulans {{Light-Harvesting Complex II}}},author={Nguyen, Hoang Long and Do, Thanh Nhut and Akhtar, Parveen and Jansen, Thomas L. C. and Knoester, Jasper and Wang, Wenda and Shen, Jian-Ren and Lambrev, Petar H. and Tan, Howe-Siang},year={2021},journal={The Journal of Physical Chemistry B},volume={125},number={4},pages={1134--1143},issn={1520-6106 1520-5207},doi={10.1021/acs.jpcb.0c10634},}
2020
Observing the Fluctuation Dynamics of Dative Bonds Using Two-Dimensional Electronic Spectroscopy
Thanh Nhut Do, Jamie Hung Ni Sim, Hoang Long Nguyen, and 2 more authors
@article{do_observing_2020,type={Journal {{Article}}},title={Observing the {{Fluctuation Dynamics}} of {{Dative Bonds Using Two-Dimensional Electronic Spectroscopy}}},author={Do, Thanh Nhut and Sim, Jamie Hung Ni and Nguyen, Hoang Long and Lu, Yunpeng and Tan, Howe-Siang},year={2020},journal={The Journal of Physical Chemistry Letters},volume={12},number={1},pages={165--170},issn={1948-7185 1948-7185},doi={10.1021/acs.jpclett.0c03243},}
Hierarchical Equations of Motion Simulation of Temperature-Dependent Two-Dimensional Electronic Spectroscopy of the ChlorophyllaManifold in LHCII
Xuan Leng, Thanh Nhut Do, Parveen Akhtar, and 3 more authors
@article{leng_hierarchical_2020,type={Journal {{Article}}},title={Hierarchical {{Equations}} of {{Motion Simulation}} of {{Temperature}}-{{Dependent Two}}-{{Dimensional Electronic Spectroscopy}} of the {{ChlorophyllaManifold}} in {{LHCII}}},author={Leng, Xuan and Do, Thanh Nhut and Akhtar, Parveen and Nguyen, Hoang Long and Lambrev, Petar H. and Tan, Howe-Siang},year={2020},journal={Chemistry -- An Asian Journal},volume={15},number={13},pages={1996--2004},issn={1861-4728 1861-471X},doi={10.1002/asia.202000467},}
2019
Revealing the Excitation Energy Transfer Network of Light-Harvesting Complex II by a Phenomenological Analysis of Two-Dimensional Electronic Spectra at 77 K
Thanh Nhut Do, Adriana Huerta-Viga, Parveen Akhtar, and 5 more authors
@article{do_revealing_2019,type={Journal {{Article}}},title={Revealing the Excitation Energy Transfer Network of {{Light-Harvesting Complex II}} by a Phenomenological Analysis of Two-Dimensional Electronic Spectra at 77 {{K}}},author={Do, Thanh Nhut and {Huerta-Viga}, Adriana and Akhtar, Parveen and Nguyen, Hoang Long and Nowakowski, Pawe{\l} J. and Khyasudeen, M. Faisal and Lambrev, Petar H. and Tan, Howe-Siang},year={2019},journal={The Journal of Chemical Physics},volume={151},number={20},pages={205101},issn={0021-9606 1089-7690},doi={10.1063/1.5125744},}
Studying the Spectral Diffusion Dynamics of Chlorophyll a and Chlorophyll b Using Two-Dimensional Electronic Spectroscopy
M. Faisal Khyasudeen, Paweł J. Nowakowski, Hoang Long Nguyen, and 3 more authors
@article{khyasudeen_studying_2019,type={Journal {{Article}}},title={Studying the Spectral Diffusion Dynamics of Chlorophyll a and Chlorophyll b Using Two-Dimensional Electronic Spectroscopy},author={Khyasudeen, M. Faisal and Nowakowski, Pawe{\l} J. and Nguyen, Hoang Long and Sim, Jamie H. N. and Do, Thanh Nhut and Tan, Howe-Siang},year={2019},journal={Chemical Physics},volume={527},issn={03010104},doi={10.1016/j.chemphys.2019.110480},}
